Analysis Note

ControlRetina

Application

This Anti-Opsin Antibody, blue is validated for use in IH(P) for the detection of Opsin.

Research CategoryNeuroscience

Research Sub CategorySensory & PNS

Immunohistochemistry: 1:200-1:300 on formalin-fixed, paraffin-embedded mouse retina tissue. Antigen retrieval method recommend is HIER with steam heat; other fixation and retrieval methods are untested.

Optimal working dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The full range of color discrimination in humans is based on the presence and function of three cone photoreceptor mechanisms. Each cone type possesses a photo-sensitive pigment-protein complex consisting of 11-cis retinal and a unique opsin protein, which gives sensitivity in the short (S cone, peak sensitivity about 420nm), middle (M cone, peak sensitivity about 530nm with polymorphism; Winderckx et al., 1993; Neitz & Neitz, 1998), and long (L cone, peak sensitivity about 560nm with polymorphism; Neitz & Jacobs, 1990) wavelengths of the light spectrum. All three opsins are transmembrane proteins with seven membrane-spanning regions. Genes for the three types of cone opsins and the rod photoreceptor rhodopsin gene seem to be homologous with varying amounts of conservation. Strongest conservation is between the middle (green) and long (red) wavelength sensitive pigments on the X chromosome, suggesting a relatively recent duplication/divergence event (Nathans, 1989; Nathans et al., 1992). The S cone (blue) opsin is located on chromosome 7 and seems to have stronger conservation with rhodopsin. Cone photoreceptor distribution in humans is dominated by the M and L cone pigments.

Immunogen

Epitope: blue

Recombinant human blue opsin.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Protein A purified

Format: Purified

Purified immunoglobulinin PBS {0.02M phosphate, 0.25M NaCl, pH 7.6} with 0.1% sodium azide as a preservative

Specificity

Recognizes Opsin, blue.

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment.